Prune Agar

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

Prune Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................17.0g
Lactose..........................................................................................5.0g
Prunes............................................................................................5.0g
Yeast extract..................................................................................1.0g
pH 5.8–6.0 at 25°C
Preparation of Medium: Add prunes to 1.0L of distilled/deionized
water. Gently heat and bring to boiling. Continue boiling for 30 min.
Pass the prunes and liquid through a sieve to include as much of the
prune pulp as possible. Add agar. Gently heat and bring to boiling. Re-
move from heat and add the lactose and yeast extract. Mix thoroughly.
Adjust pH to 5.8–6.0. Distribute into tubes or flasks. Autoclave for 20
min at 20 psi pressure–126°C. Pour into sterile Petri dishes or leave in
tubes.
Use: For the cultivation and maintenance of Pyricularia oryzae.