PSA (Potato Sucrose Agar)

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

PSA
(Potato Sucrose Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potatoes.....................................................................................200.0g
Agar............................................................................................20.0g
Sucrose........................................................................................20.0g
Preparation of Medium: Slice potatoes with skin. Place 200.0g of
potatoes in 1.0L of distilled/deionized water. Gently heat and bring to
boiling. Allow to boil for 20 min. Filter through Whatman filter paper.
Add agar and sucrose to filtrate. Bring volume to 1.0L with distilled/
deionized water. Mix thoroughly. Gently heat and bring to boiling. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of Aspergillus oryzae, Cylindrodendrum
album, Nectria ipomoeae, Nectria haematococca, and other fungi.