Pseudoalteromonas spiralis Medium (DSMZ Medium 1072)

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudoalteromonas spiralis Medium
(DSMZ Medium 1072)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................15.0g
Na-acetate.....................................................................................1.0g
Yeast extract..................................................................................0.5g
Casamino acids.............................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
KH 2 PO 4 .........................................................................................0.3g
NH 4 Cl ..........................................................................................0.3g
KCl................................................................................................0.3g
CaCl 2 ·2H 2 O ................................................................................0.05g
Trace elements solution ............................................................2.0mL
Vitamin solution.........................................................................2.0mL
pH 7.8 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MnCl 2 ·4H 2 O ................................................................................ 0.3g
FeSO 4 ·7H 2 O...............................................................................3.0mg
Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Vitamin Solution:

Composition per liter:
Thiamine-HCl·2H 2 O..............................................................400.0mg
Nicotinic acid.........................................................................400.0mg
Biotin .......................................................................................80.0mg
Vitamin B 12 ..............................................................................20.0mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 5.9. Distribute into tubes or flasks. Gently heat
while stirring and bring to boiling. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to room termperature. Aseptically
add vitamin solution. Mix thorougly. Adjust to pH 7.8 with NaHCO 3 .
Aseptically distribute into culture vessels.
Use:  For the cultivation of Pseudoalteromonas spiralis.