Pseudomonas halophila Medium

[所属分类:培养基配方] [发布时间:2021-7-15] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas halophila Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................890.0mL
Solution B..............................................................................100.0mL
Vitamin solution.......................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Solution A:
Composition per 890.0mL:
NaCl............................................................................................46.8g
MgSO 4 ·7H 2 O..............................................................................39.4g
Glycerol ........................................................................................5.0g
NH 4 Cl ...........................................................................................1.0g
Trace elements solution SL-10 ..................................................1.0mL
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ................................................................................0.19g
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-10:  Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly.
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 890.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to room temperature.
Solution B:
Composition per 100.0mL:
KH 2 PO 4 .........................................................................................1.0g
Preparation of Solution B:  Add KH 2 PO 4 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to room temperature.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
Calcium pantothenate ................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
Robiflavin ..................................................................................5.0mg
Thiamine·HCl ............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
p-Aminobenzoic acid.................................................................1.0mg
Cyanocobalamin ......................................................................0.01mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Aseptically combine 890.0mL of cooled
sterile solution A, 100.0mL of cooled sterile solution B, and 10.0mL of
sterile vitamin solution. Mix thoroughly. Adjust pH to 7.0 with sterile
6N NaOH or HCl solution.
Use:  For the cultivation and maintenance of Pseudomonas halophila .