Pseudomonas HiVeg Agar Base with Glycerol and Selective Supplement

[所属分类:培养基配方] [发布时间:2021-7-15] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas HiVeg Agar Base
with Glycerol and Selective Supplement

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone No. 2.....................................................................16.0g
Agar............................................................................................ 11.0g
Plant hydrolysate ........................................................................10.0g
K 2 SO 4 .........................................................................................10.0g
MnCl 2 , anhydrous.........................................................................1.4g
Glycerol ...................................................................................10.0mL
Selective supplement...............................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without glycerol or selective supplement, is
available as a premixed powder from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Cetrimide......................................................................................0.2g
Nalidixic acid...........................................................................15.0mg
Preparation of Selective Supplement Solution: Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add glycerol and then other components,
except selective supplement, to distilled/deionized water and bring vol-
ume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 10.0mL selective supple-
ment. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the selective isolation of Pseudomonas species.