Pseudomonas Medium (ATCC Medium 59)

[所属分类:培养基配方] [发布时间:2021-7-15] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas Medium
(ATCC Medium 59)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

K 2 HPO 4 .......................................................................................1.15g
NH 4 NO 3 ........................................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .....................................................................................0.625g
MgSO 4 ·7H 2 O..............................................................................0.02g
Pyrrolidine.................................................................................4.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add pyrrolidine to 500.0mL of distilled/
deionized water. Mix thoroughly. Adjust pH to 7.0. Add remaining
components. Bring volume to 1.0L with distilled/deionized water. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use:  For the cultivation and maintenance of Pseudomonas fluore-
scens.