Pseudomonas Medium (ATCC Medium 179)

[所属分类:培养基配方] [发布时间:2021-7-15] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas Medium
(ATCC Medium 179)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1020.0mL:

Solution 1......................................................................................1.0L
Solution 3.................................................................................15.0mL
Solution 2...................................................................................5.0mL
pH 6.8 ± 0.2 at 25°C
Solution 1:
Composition per liter:
Agar............................................................................................20.0g
K 2 HPO 4 .......................................................................................2.56g
KH 2 PO 4 .......................................................................................2.08g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
Preparation of Solution 1: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 6.8. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution 2:
Composition per 100.0mL:
Ferric ammonium citrate...............................................................1.0g
CaCl 2 .............................................................................................0.1g
Preparation of Solution 2:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Solution 3:
Composition per 100.0mL:
Succinic acid............................................................................... 11.8g
Preparation of Solution 3:  Add succinic acid to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 6.0
with NaOH. Filter sterilize.
Preparation of Medium: To 1.0L of cooled sterile solution 1, asep-
tically add 5.0mL of sterile solution 2 and 15.0mL of sterile solution 3.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use:  For the cultivation and maintenance of Pseudomonas lemoignei
and Pseudomonas putida.