Purple Lactose Agar

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]

Purple Lactose Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................10.0g
Lactose........................................................................................10.0g
Peptone .........................................................................................5.0g
Beef extract...................................................................................3.0g
Bromcresol Purple....................................................................0.025g
pH 6.8 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Al-
low tubes to cool in a slanted position.
Use: For the detection and differentiation of members of the Enter-
obacteriaceae. Bacteria that can ferment lactose turn the medium yel-
low.