Purple Serum Agar Base

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]

Purple Serum Agar Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Lactose........................................................................................20.0g
Peptone........................................................................................20.0g
NaCl..............................................................................................5.0g
Bromcresol Purple ......................................................................0.03g
Phenol Red................................................................................0.024g
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and differentiation of Gram-negative bacteria
isolated from the urinary tract. Bacteria that can ferment lactose turn
the medium yellow.