PV Blood Agar (Paromomycin Vancomycin Blood Agar)

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]

PV Blood Agar
(Paromomycin Vancomycin Blood Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Pancreatic digest of casein..........................................................15.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................5.0g
Yeast extract..................................................................................5.0g
L- Cystine .......................................................................................0.4g
Paromomycin................................................................................0.1g
Vancomycin ...............................................................................7.5mg
Hemin.........................................................................................5.0mg
Sheep blood, defibrinated........................................................50.0mL
Vitamin K 1 solution .................................................................10.0mL
pH 7.5 ± 0.2 at 25°C
Vitamin K 1 Solution:
Composition per 10.0mL:
Vitamin K 1 ..................................................................................0.01g
Ethanol.....................................................................................10.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 10.0mL
of absolute ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components—except vitamin K 1 so-
lution, sheep blood, paromomycin, and vancomycin—to distilled/de-
ionized water and bring volume to 940.0mL. Mix thoroughly. Gently
heat and bring to boiling for 1 min. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°–55°C. Aseptically add the sterile vitamin K 1
solution, sheep blood, vancomycin, and paromomycin. Mix thorough-
ly. Pour into sterile Petri dishes.
Use: For the selective cultivation of fastidious anaerobic bacteria.