PW Medium (LMG 182)

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]

PW Medium
(LMG 182)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Agar ............................................................................................12.0g
Papaic digest of soybean meal......................................................4.0g
KH 2 PO 4 .........................................................................................1.2g
K 2 HPO 4 .........................................................................................1.0g
Trypticase™ peptone....................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.4g
Glutamine solution...................................................................50.0mL
Bovine serum albumin solution...............................................30.0mL
Phenol Red (0.2% solution).....................................................10.0mL
Solution A................................................................................10.0mL
Glutamine Solution:
Composition per 50.0mL:
L -Glutamine..................................................................................4.0g
Preparation of Glutamine Solution: Add glutamine to distilled/
deionized water and bring volume to 50.0ml. Mix thoroughly. Filter
sterilize.
Bovine Serum Albumin Solution:
Composition per 50.0mL:
Bovine serum albumin, fraction V..............................................10.0g
Preparation of Bovine Serum Albumin Solution: Add bovine
serum albumin to distilled/deionized water and bring volume to
50.0mL. Mix thoroughly. Filter sterilize.
Solution A:
Composition per 101.0mL:
NaOH (0.05N solution)..........................................................100.0mL
Hemin chloride .............................................................................0.1g
Preparation of Medium: Add components, except glutamine solu-
tion and bovine serum albumin solution, to distilled/deionized water
and bring volume to 920.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C. Aseptically add 50.0mL of sterile glutamine solution and
10.0mL of sterile bovine serum albumin solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Xylella fastidiosa.