PY Basal Medium

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]

PY Basal Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 104.0mL:

Yeast extract..................................................................................1.0g
L -Cysteine·HCl·H 2 O.....................................................................0.5g
Peptone .........................................................................................0.5g
Pancreatic digest of casein............................................................0.5g
Resazurin .................................................................................0.16mg
Salts solution..............................................................................4.0mL
Salts Solution:
Composition per liter:
NaHCO 3 ......................................................................................10.0g
NaCl..............................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
CaCl 2 .............................................................................................0.2g
MgSO 4 ..........................................................................................0.2g
Preparation of Salts Solution: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 104.0mL. Mix thoroughly. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the identification of treponemes.