PYb Agar

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]

PYb Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Proteose peptone...........................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 solution......................................................................32.0mL
Na 2 HPO 4 solution......................................................................8.0mL
CaCl 2 solution............................................................................4.0mL
MgSO 4 ·7H 2 O solution...............................................................2.5mL
pH 6.5 ± 0.5 at 25°C
CaCl 2 Solution:
Composition per 100.0mL:
CaCl 2 ...........................................................................................0.75g
Preparation of CaCl 2 Solution: Add CaCl 2 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 6.5.
Autoclave for 25 min at 15 psi pressure–121°C. Cool to 50°–55°C.
MgSO 4 ·7H 2 O Solution:
Composition per 100.0mL:
MgSO 4 ·7H 2 O................................................................................9.8g
Preparation of MgSO 4 ·7H 2 O Solution: Add MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Adjust pH to 6.5. Autoclave for 25 min at 15 psi pressure–121°C.
Cool to 50°–55°C.
Na 2 HPO 4 Solution:
Composition per 100.0mL:
Na 2 HPO 4 .......................................................................................6.7g
Preparation of Na 2 HPO 4 Solution: Add Na 2 HPO 4 to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust
pH to 6.5. Autoclave for 25 min at 15 psi pressure–121°C. Cool to 50°–
55°C.
KH 2 PO 4 Solution:
Composition per 100.0mL:
KH 2 PO 4 .........................................................................................3.4g
Preparation of KH 2 PO 4 Solution: Add KH 2 PO 4 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Adjust
pH to 6.5. Autoclave for 25 min at 15 psi pressure–121°C. Cool to 50°–
55°C.
Preparation of Medium: Add components, except KH 2 PO 4 solu-
tion, Na 2 HPO 4 solution, CaCl 2 solution, and MgSO 4 ·7H 2 O solution, to
distilled/deionized water and bring volume to 953.5mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 32.0mL of sterile
KH 2 PO 4  solution, 8.0mL of sterile Na 2 HPO 4 solution, 4.0mL of sterile
CaCl 2 solution, and 2.5mL of sterile MgSO 4 ·7H 2 O solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Acanthamoeba hatchetti, Acanthamoeba
jacobsi, Acanthamoeba polyphaga, Echinamoeba exundans, Naegle-
ria gruberi, Paratetramitus jugosus, Rhizamoeba species, Tetramitus
rostratus, and Vahlkampfia lobospinosa.