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PYCS Medium

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]
PYCS Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

KH 2 PO 4 .........................................................................................1.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
MgCl 2 ·6H 2 O .................................................................................0.2g
NaCl..............................................................................................0.2g
CaCl 2 ·2H 2 O .............................................................................45.0mg
Fructose solution......................................................................50.0mL
NaHCO 3 solution.....................................................................10.0mL
Wolfe’s mineral solution..........................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Fructose Solution:
Composition per 50.0mL:
D -Fructose.....................................................................................5.0g
Preparation of Fructose Solution:  Add  D -fructose to distilled/de-
ionized water and bring volume to 50.0mL. Mix thoroughly. Filter ster-
ilize.
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO 3 ......................................................................................1.68g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Wolfe’s Mineral Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·H 2 O..................................................................................0.5g
CaCl 2 .............................................................................................0.1g
CoCl 2 ·6H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
AlK(SO 4 ) 2 ·12H 2 O.......................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.01g
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to approximately 500.0mL of water and adjust to pH 6.5 with
KOH to dissolve the compound. Bring volume to 1.0L with remaining
water and add remaining components one at a time.
Preparation of Medium: Add components, except fructose solu-
tion and NaHCO 3 solution, to distilled/deionized water and bring vol-
ume to 940.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 50.0mL of sterile fructose solution
and 10.0mL of sterile NaHCO 3 solution. Mix thoroughly. Adjust pH to
7.2. Aseptically distribute into sterile tubes or flasks. Fill containers to
capacity.
Use: For the cultivation of Rhodoferax fermentans and Thiocapsa
halophila.
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