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PYE Medium

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]
PYE Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract..................................................................................4.0g
Sodium pyruvate...........................................................................2.2g
K 2 HPO 4 .........................................................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Na 2 S 2 O 3 ·5H 2 O..............................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.02g
Trace elements solution SL-6 ....................................................1.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Heliobacterium modestocaldum.
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