PYG Agar

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYG Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Proteose peptone.........................................................................20.0g
Yeast extract..................................................................................1.0g
Glucose solution ......................................................................50.0mL
Sodium citrate solution............................................................34.0mL
Ferric ammonium sulfate.........................................................10.0mL
KH 2 PO 4 solution......................................................................10.0mL
MgSO 4 ·7H 2 O solution.............................................................10.0mL
Na 2 HPO 4 solution....................................................................10.0mL
CaCl 2 solution............................................................................8.0mL
pH 6.5 ± 0.5 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose.......................................................................................36.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize. Warm to 55°C.
MgSO 4 ·7H 2 O Solution:
Composition per 100.0mL:
MgSO 4 ·7H 2 O................................................................................9.8g
Preparation of MgSO 4 ·7H 2 O Solution: Add MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Adjust pH to 6.5. Autoclave for 25 min at 15 psi pressure–121°C.
Cool to 50°–55°C.
Ferric Ammonium Sulfate Solution:
Composition per 100.0mL:
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O..............................................................0.135g
Preparation of Ferric Ammonium Sulfate Solution: Add
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O to distilled/deionized water and bring volume
to 100.0mL. Mix thoroughly. Adjust pH to 6.5. Autoclave for 25 min
at 15 psi pressure–121°C. Cool to 50°–55°C.
Na 2 HPO 4 Solution:
Composition per 100.0mL:
Na 2 HPO 4 .......................................................................................6.7g
Preparation of Na 2 HPO 4 Solution: Add Na 2 HPO 4 to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust
pH to 6.5. Autoclave for 25 min at 15 psi pressure–121°C. Cool to 50°–
55°C.
Sodium Citrate Solution:
Composition per 100.0mL:
Sodium citrate·2H 2 O.....................................................................2.9g
Preparation of Sodium Citrate Solution: Add 2.9g of sodium
citrate·2H 2 O to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Adjust pH to 6.5. Autoclave for 25 min at
15 psi pressure–121°C. Cool to 50°–55°C.
KH 2 PO 4 Solution:
Composition per 100.0mL:
KH 2 PO 4 .........................................................................................3.4g
Preparation of KH 2 PO 4 Solution: Add KH 2 PO 4 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Adjust
pH to 6.5. Autoclave for 25 min at 15 psi pressure–121°C. Cool to 50°–
55°C.
CaCl 2 Solution:
Composition per 100.0mL:
CaCl 2 ...........................................................................................0.75g
Preparation of CaCl 2 Solution: Add CaCl 2 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 6.5.
Autoclave for 25 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Preparation of Medium: Add components, except glucose solution,
sodium citrate solution, ferric ammonium sulfate solution, KH 2 PO 4 solu-
tion, Na 2 HPO 4 solution, CaCl 2 solution, and MgSO 4 ·7H 2 O solution, to
distilled/deionized water and bring volume to 868.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Aseptically add 50.0mL of sterile glucose solu-
tion, 34.0mL of sterile sodium citrate solution, 10.0mL of sterile ferric am-
monium sulfate solution, 10.0mL of sterile KH 2 PO 4 solution, 10.0mL of
sterile Na 2 HPO 4 solution, 8.0mL of sterile CaCl 2 solution, and 10.0mL of
sterile MgSO 4 ·7H 2 O solution. Mix thoroughly. Pour into sterile Petri dish-
es or distribute into sterile tubes.
Use: For the cultivation of Acanthamoeba astronyxis, Acanthamoeba
castellanii, Acanthamoeba comandoni, Acanthamoeba culbertsoni,
Acanthamoeba divionensis, Acanthamoeba healyi, Acanthamoeba
quina, Acanthamoeba lenticulata, Acanthamoeba lugdunensis, Acan-
thamoeba mauritaniensis, Acanthamoeba palestinensis, Acanthamoeba
pearcei, Acanthamoeba polyphaga, Acanthamoeba pustulosa, Acan-
thamoeba rhysodes, Acanthamoeba royreba, Acanthamoeba species,
Acanthamoeba terricola, and Acanthamoeba triangularis.