PYG Broth (Peptone Yeast Extract Glucose Broth)

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYG Broth
(Peptone Yeast Extract Glucose Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................20.0g
D -Glucose....................................................................................10.0g
Yeast extract................................................................................10.0g
L -cysteine·HCl·H 2 O......................................................................0.5g
VPI salt solution.......................................................................40.0mL
Resazurin solution......................................................................4.0mL
pH 7.2 ± 0.2 at 25°C
Resazurin Solution:
Composition per 44.0mL:
Resazurin ..................................................................................0.044g
Preparation of Resazurin Solution: Add resazurin to distilled/
deionized water and bring volume to 44.0mL. Mix thoroughly.
VPI Salt Solution:
Composition per 40.0mL:
CaCl 2 .............................................................................................0.2g
MgSO 4 ..........................................................................................0.2g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Preparation of VPI Salt Solution:  Add CaCl 2 and MgSO 4 to
300.0mL of distilled/deionized water. Mix thoroughly until dissolved.
Bring volume to 800.0mL with distilled/deionized water. Add remain-
ing components while stirring. Bring volume to 1.0L. Mix thoroughly.
Store at 4°C.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into screw-
capped tubes in 7.0mL volumes. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C under 100% N 2 .
Use: For the cultivation of a wide variety of anaerobic bacteria.