PYG Medium (Peptone Yeast Extract Glucose Medium)

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYG Medium
(Peptone Yeast Extract Glucose Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone.........................................................................20.0g
Glucose.......................................................................................18.0g
Yeast extract..................................................................................2.0g
Sodium citrate·2H 2 O.....................................................................1.0g
MgSO 4 ·7H 2 O..............................................................................0.98g
Na 2 HPO 4 ·7H 2 O.........................................................................0.355g
KH 2 PO 4 .......................................................................................0.34g
CaCl 2 .........................................................................................0.059g
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O................................................................0.02g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components, except CaCl 2 , to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly
until dissolved. Add CaCl 2 . Mix thoroughly. Bring volume to 1.0L
with distilled/deionized water. Distribute into screw-capped tubes in
5.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Acanthamoeba species.