PYG Medium (Peptone Yeast Extract Glucose Medium)

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYG Medium
(Peptone Yeast Extract Glucose Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Glucose.......................................................................................0.25g
Peptone .......................................................................................0.25g
Yeast extract................................................................................0.25g
Hutner’s modified salt solution ...............................................20.0mL
Vitamin solution.......................................................................10.0mL
Hutner’s Modified Salts Solution:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O ................................................................................3.34g
FeSO 4 ·7H 2 O..................................................................................0.1g
Metals “44”..............................................................................50.0mL
Preparation of Hutner’s Modified Salts Solution: Add nitrilo-
triacetic acid to 500.0mL of distilled/deionized water. Dissolve by ad-
justing pH to 6.5 with KOH. Add remaining components. Add
distilled/deionized water to 1.0L.
Metals “44”:
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.................................................................................1.1g
FeSO 4 ·7H 2 O..................................................................................0.5g
EDTA..........................................................................................0.25g
MnSO 4 ·7H 2 O............................................................................0.154g
CuSO 4 ·5H 2 O...............................................................................0.04g
Co(NO 3 ) 2 ·6H 2 O........................................................................0.025g
Na 2 B 4 O 7 ·10H 2 O........................................................................0.018g
Preparation of Metals “44”: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Vitamin Solution:
Composition per liter :
Pyridoxine·HCl...........................................................................0.01g
Calcium pantothenate ................................................................5.0mg
Nicotinamide..............................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Thiamine·HCl ............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile vitamin solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the isolation and cultivation of Pasteuria ramosa.