PYG Medium (B) (DSMZ Medium 1139)

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYG Medium (B)
(DSMZ Medium 1139)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract ...............................................................................10.0g
Glucose ......................................................................................10.0g
Polypeptone ..................................................................................5.0g
Tryptone .......................................................................................5.0g
Salts solution ...........................................................................40.0mL
pH 7.2 ± 0.2 at 25°C
Salts Solution:
Composition per 10.0mL:
NaHCO 3 ......................................................................................10.0g
NaCl..............................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 · 7H 2 O................................................................................0.4g
CaC l 2 ·2H 2 O...................................................................................0.2g
Preparation of Salts Solution:  Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C.
Use:  For the cultivation of Flavobacterium omnivorum.