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PYG Medium (E) (DSMZ Medium 1140)

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]
PYG Medium (E)
(DSMZ Medium 1140)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ...........................................................................................15.0g
Peptone ........................................................................................5.0g
Beef extract ..................................................................................3.0g
MgSO 4 · 7H 2 O................................................................................1.5g
Yeast extract .................................................................................0.2g
Glucose ........................................................................................5.0g
NaCl .............................................................................................0.5g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Pour into Petri dishes or leave in tubes.
Use:  For the cultivation of Flavobacterium glaciei.
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