PYG Medium, Modified

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYG Medium, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 961.0mL:

Yeast extract................................................................................10.0g
Beef extract...................................................................................5.0g
Glucose.........................................................................................5.0g
Peptone .........................................................................................5.0g
Trypticase™..................................................................................5.0g
K 2 HPO 4 .........................................................................................2.0g
L -Cysteine·HCl .............................................................................0.5g
Resazurin ...................................................................................1.0mg
Salt solution.............................................................................40.0mL
Hemin solution.........................................................................10.0mL
Tween™ 80................................................................................1.0mL
Vitamin K 1 solution...................................................................0.2mL
pH 7.2 ± 0.2 at 25°C
Salt Solution:
Composition per liter:
NaHCO 3 ......................................................................................10.0g
NaCl..............................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O............................................................................... 0.5g
CaCl 2 ·2H 2 O ................................................................................0.25g
Preparation of Salt Solution : Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Hemin Solution:
Composition per 100.0mL:
Hemin ......................................................................................50.0mg
NaOH (1N solution)...................................................................1.0mL
Preparation of Hemin Solution : Add hemin to 1.0mL of NaOH
solution. Mix thoroughly until dissolved. Bring volume to 100.0mL
with distilled/deionized water. Mix thoroughly. Store at 4°C.
Vitamin K 1 Solution:
Composition per 20.0mL:
Vitamin K 1 ....................................................................................0.1g
Ethanol (95% solution)............................................................20.0mL
Preparation of Vitamin K 1 Solution : Add vitamin K 1 to 95% eth-
anol and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.
Store in a brown glass bottle at 4°C.
Preparation of Medium: Add components, except  L -cysteine·HCl,
vitamin K 1 solution, and hemin solution, to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Continue boiling for 5 min. Cool to room temperature while
sparging with 100% CO 2 . Add  L -cysteine·HCl, vitamin K 1 solution,
and hemin solution. Mix thoroughly. Adjust pH to 7.2 using 8N NaOH
while continuing to sparge with 100% CO 2 . After pH has been attained
sparge with 100% N 2 . Anaerobically distribute into tubes or flasks. Au-
toclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Acetomicrobium flavidum, Actinomyces
denticolens, Actinomyces hordeovulneris, Actinomyces meyeri, Actin-
omyces pyogenes, Anaerobiospirillum succiniciproducens, Arcano-
bacterium haemolyticum, Atopobium minutum, Atopobium parvulum,
Atopobium rimae, Bacteroides eggerthii, Bacteroides helcogenes,
Bacteroides pyogenes, Bacteroides species, Bacteroides suis, Bacte-
roides uniformis, Bifidobacterium gallinarum, Clostridium beijer-
inckii, Clostridium butyricum, Clostridium species, Clostridium
xylanolyticum, Coriobacterium glomerans, Eubacterium combesii,
Eubacterium limosum, Eubacterium multiforme, Eubacterium nitrito-
genes, Eubacterium tenue, Fusobacterium naviforme, Fusobacterium
necrophorum, Fusobacterium nucleatum, Gemella morbillorum, Lac-
tobacillus catenaformis, Lactobacillus crispatus, Megamonas hyper-
megas, Megasphaera cerevisiae, Megasphaera elsdenii, Mitsuokella
multiacidus, Peptostreptococcus anaerobius, Peptostreptococcus
indolicus, Peptostreptococcus magnus, Peptostreptococcus prevotii,
Peptostreptococcus productus, Peptostreptococcus species, Pepto-
streptococcus tetradius, Prevotella bivia, Prevotella buccae, Pre-
votella buccalis, Prevotella denticola, Prevotella disiens, Prevotella
intermedia, Propionibacterium acnes, Propionibacterium avidum,
Propionibacterium freudenreichii, Propionibacterium granulosum,
Propionibacterium lymphophilum, Selenomonas sputigena, Staphylo-
coccus saccharolyticus, Streptococcus constellatus, Streptococcus
hansenii, Streptococcus intermedius, and Streptococcus pleomorphus.