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PYG with 0.1% Tween™ 80

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]
PYG with 0.1% Tween™ 80

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1004.2mL:

Glucose.......................................................................................10.0g
Yeast extract................................................................................10.0g
Peptone ........................................................................................ 5.0g
Pancreatic digest of casein........................................................... 5.0g
Tween™ 80...................................................................................1.0g
L -Cysteine·HCl·H 2 O .....................................................................0.5g
Salts solution........................................................................... 40.0mL
Hemin solution.........................................................................10.0mL
Resazurin (0.025% solution)......................................................4.0mL
Vitamin K 1 solution ...................................................................0.2mL
pH 7.0 ± 0.2 at 25°C
Salts Solution:
Composition per liter:
NaHCO 3 ......................................................................................10.0g
NaCl..............................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
CaCl 2  , anhydrous .........................................................................0.2g
MgSO 4 ..........................................................................................0.2g
Preparation of Salts Solution: Add CaCl 2 and MgSO 4 to distilled/
deionized water and bring volume to 300.0mL. Add 500.0mL of dis-
tilled/deionized water. Add the remaining components while swirling
slowly. Add 200.0mL of distilled/deionized water. Mix thoroughly.
Store at 4°C.
Hemin Solution:
Composition per 100.0mL:
Hemin.......................................................................................50.0mg
NaOH (1N solution)...................................................................1.0mL
Preparation of Hemin Solution: Dissolve hemin in 1.0mL of 1N
NaOH solution. Bring volume to 100.0mL with distilled/deionized wa-
ter. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Vitamin K 1 Solution:
Composition per 30.15mL:
Ethanol (95% solution)............................................................30.0mL
Vitamin K 1 ...............................................................................0.15mL
Preparation of Vitamin K 1 Solution: Combine components. Mix
thoroughly. Store at 4°C in the dark. Discard solution after 1 month.
Preparation of Medium: Add components, except the 0.5g of  L -cyste-
ine·HCl·H 2 O, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Add  L -
cysteine·HCl·H 2 O. Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Lactobacillus uli and Atopobium rimae.
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