Pygeye Agar

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pygeye Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Glucose .......................................................................................10.0g
Neopeptone...................................................................................5.0g
Yeast extract..................................................................................0.5g
Egg yolk emulsion, 50%........................................................200.0mL
Egg Yolk Emulsion, 50%:
Composition  per 200mL:
Chicken egg yolks............................................................................22
Whole chicken egg.............................................................................2
NaCl (0.9% solution).............................................................100.0mL
Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 2 chicken eggs. Beat
to form emulsion. Measure 100.0mL of egg yolk emulsion and add to
100.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize. Warm
to 45°–50°C.
Preparation of Medium: Add components, except egg yolk emul-
sion, 50%, to distilled/deionized water and bring volume to 800.0mL.
Mix thoroughly. Gently heat while stirring and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asepti-
cally add 200.0mL of sterile egg yolk emulsion, 50%. Mix thoroughly.
Pour into sterile Petri dishes in 15.0mL volumes.
Use: For the cultivation and maintenance of Conidiobolus apiculatus,
Conidiobolus obscurus, Dactylella oviparasitica, Entomophthora
aphidis, Entomophthora ignobilis, Entomophthora sphaerosperma,
and Erynia blunckii.