PYGHS Medium

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYGHS Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 60.0mL:

Beef heart, solids from infusion..................................................10.0g
Glucose.........................................................................................1.0g
Polypeptone™ ..............................................................................1.0g
Yeast extract..................................................................................1.0g
L -Cysteine·HCl·H 2 O.....................................................................0.9g
Ionagar No. 2..............................................................................0.72g
NaHCO 3 ........................................................................................0.5g
Tryptose........................................................................................0.2g
NaCl..............................................................................................0.1g
Resazurin .................................................................................0.16mg
Rabbit serum, inactivated ..........................................................5.0mL
Salts solution..............................................................................5.0mL
pH 7.2–7.5 at 25°C
Salts Solution:
Composition per 400.0mL:
K 2 HPO 4 .........................................................................................0.9g
NaCl..............................................................................................0.8g
KH 2 PO 4 .........................................................................................0.4g
MnCl 2 ·4H 2 O ...............................................................................0.16g
MgSO 4 ........................................................................................0.08g
Preparation of Salts Solution: Add components to distilled/deion-
ized water and bring volume to 400.0L. Mix thoroughly.
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 55.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 5.0mL of sterile
rabbit serum. Mix thoroughly.
Use: For the cultivation of treponemes.