PYGM Medium (Peptone Yeast Extract Glucose Maltose Medium)

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYGM Medium
(Peptone Yeast Extract Glucose Maltose Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 261.0mL:

Peptone .........................................................................................5.0g
Yeast extract..................................................................................2.5g
Glucose.......................................................................................1.25g
Maltose .......................................................................................1.25g
L -Cysteine·HCl·H 2 O.................................................................0.125g
Salts solution............................................................................10.0mL
Resazurin (0.025% solution) .....................................................1.0mL
Salts Solution:
Composition per 100.0mL:
NaHCO 3 ........................................................................................1.0g
NaCl..............................................................................................0.2g
K 2 HPO 4 .........................................................................................0.1g
KH 2 PO 4 .........................................................................................0.1g
CaCl 2 , anhydrous........................................................................0.02g
MgSO 4 ........................................................................................0.02g
H 2 SO 4 (50% solution)................................................................0.3mL
Na 2 MoO 4 ·2H 2 O ..........................................................................1.0μg
CoCl 2 ·6H 2 O ................................................................................1.0μg
Preparation of Salts Solution: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 261.0mL. Mix thoroughly. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Bacteroides praeacutus, Eubacterium nitri-
togenes, Lactobacillus ruminis, and Tissierella praeacuta.