PYGV Medium (Peptone Yeast Extract Glucose Vitamin Medium)

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYGV Medium
(Peptone Yeast Extract Glucose Vitamin Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone........................................................................................0.25g
Yeast extract................................................................................0.25g
Mineral salt solution ................................................................20.0mL
Glucose solution ......................................................................10.0mL
Vitamin solution.........................................................................5.0mL
pH 7.5 ± 0.2 at 25°C
Mineral Salt Solution:
Composition per liter:
MgSO 4· 7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O ................................................................................3.34g
FeSO 4 ·7H 2 O.............................................................................99.0mg
Na 2 MoO 4 ·2H 2 O.....................................................................12.67mg
Metals “44”..............................................................................50.0mL
Preparation of Mineral Salt Solution: Add nitrilotriacetic acid
to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to
6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Readjust pH to 7.2.
Metals “44”:
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.................................................................................1.1g
FeSO 4 ·7H 2 O..................................................................................0.5g
EDTA..........................................................................................0.25g
MnSO 4 ·7H 2 O............................................................................0.154g
CuSO 4 ·5H 2 O...............................................................................0.04g
Co(NO 3 ) 2 ·6H 2 O........................................................................0.025g
Na 2 B 4 O 7 ·10H 2 O........................................................................0.018g
Preparation of Metals “44”: Add a few drops of H 2 SO 4 to dis-
tilled/deionized water to inhibit precipitate formation. Add compo-
nents to acidified distilled/deionized water and bring volume to
100.0mL. Mix thoroughly.
Glucose Solution:
Composition per 100.0mL:
D -Glucose......................................................................................2.5g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl...........................................................................0.02g
p-Aminobenzoic acid..................................................................0.01g
Calcium  D -pantothenate..............................................................0.01g
Nicotinamide...............................................................................0.01g
Riboflavin...................................................................................0.01g
Thiamine·HCl .............................................................................0.01g
Biotin.........................................................................................4.0mg
Folic acid ...................................................................................4.0mg
Cyanocobalamin........................................................................0.2mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except glucose solu-
tion and vitamin solution, to distilled/deionized water and bring vol-
ume to 985.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of sterile glucose solution and 5.0mL of sterile
vitamin solution. Mix thoroughly. Adjust pH to 7.5 with sterile KOH if
necessary. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Blastobacter aggregatus, Blas-
tobacter capsulatus, Blastobacter denitrificans, and Planctomyces limno-
philus.