PYGV Medium

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYGV Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone........................................................................................0.25g
Yeast extract................................................................................0.25g
Mineral solution.......................................................................20.0mL
Glucose solution ......................................................................10.0mL
Vitamin solution.........................................................................5.0mL
pH 7.5 ± 0.2 at 25°C
Mineral Solution:
Composition per liter:
MgSO 4 ·7H 2 0...............................................................................29.7g
NaMoO 4 ·2H 2 O..........................................................................12.67g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O.................................................................................3.34g
FeSO 4 ·7H 2 O..................................................................................0.1g
Metals “44” solution................................................................50.0mL
Preparation of Mineral Solution: Add nitrilotriacetic acid to
500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5
with KOH. Add remaining components. Readjust pH to 7.2 with
H 2 SO 4 or KOH. Add distilled/deionized water to 1.0L. Store at 5°C.
Metals “44” Solution:
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.................................................................................1.1g
FeSO 4 ·7H 2 O..................................................................................0.5g
EDTA..........................................................................................0.25g
MnSO 4 ·7H 2 O............................................................................0.154g
CuSO 4 ·5H 2 O...............................................................................0.04g
Co(NO 3 ) 2 ·6H 2 O ........................................................................0.025g
Na 2 B 4 O 7 ·10H 2 O........................................................................0.018g
Preparation of Metals “44” Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Glucose Solution:
Composition per 100.0mL:
D- Glucose......................................................................................2.5g
Preparation of Glucose Solution: Add  D- glucose to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Vitamin Solution:
Composition per liter:
Pyridoxin·HCl.............................................................................0.02g
p-Aminobenzoic acid..................................................................0.01g
Ca-panthothenate........................................................................0.01g
Nicotinamide...............................................................................0.01g
Riboflavin ...................................................................................0.01g
Thiamine·HCl .............................................................................0.01g
Biotin .........................................................................................4.0mg
Folic acid....................................................................................4.0mg
Vitamin B 12 ................................................................................0.2mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L.
Preparation of Medium: Add components, except glucose solu-
tion and vitamin solution, to distilled/deionized water and bring vol-
ume to 985.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 20 min at 15 psi pressure–121°C. Cool to 60°C. Asepti-
cally add 10.0mL of sterile glucose solution and 5.0mL of sterile vita-
min solution. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the enrichment of Stella species from polluted waters.