Pyrazinamidase Agar (BAM M131)

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pyrazinamidase Agar
(BAM M131)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein........................................................ 11.25g
Agar.......................................................................................... 11.25g
Papaic digest of soybean meal....................................................3.75g
NaCl............................................................................................3.75g
Yeast extract..................................................................................3.0g
Pyrazine-carboxamide ..................................................................1.0g
Tris maleate, 0.2M, pH6.0 ............................................................1.0L
pH 6.0 ± 0.2 at 25°C
Preparation of Medium: Add components to 0.2M tris maleate and
bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boil-
ing. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C. Pour into sterile Petri dishes or leave in tubes. For slants
allow tubes to cool in an inclined position.
Use: For the cultivation of Yersinia spp.