Pyrazinamide Medium

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pyrazinamide Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Na 2 HPO 4 .......................................................................................2.5g
Sodium pyruvate...........................................................................2.0g
L- Asparagine.................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
Pancreatic digest of casein............................................................0.5g
Tween™ 80...................................................................................0.2g
Pyrazinamide ................................................................................0.1g
CaCl 2 ·2H 2 O ...............................................................................0.5mg
CuSO 4 ·5H 2 O..............................................................................0.1mg
ZnSO 4 ·7H 2 O..............................................................................0.1mg
Ferric ammonium citrate.............................................................0.05g
MgSO 4 ·7H 2 O..............................................................................0.01g
pH 6.6 ± 0.2 at 25°C
Preparation of Medium: Combine components. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes in 5.0mL vol-
umes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to
cool in a slanted position.
Use: For the cultivation and differentiation of Corynebacterium spe-
cies and related organisms. Bacteria that produce pyrazinamidase turn
the medium pink.