Pyridine Medium

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pyridine Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

K 2 HPO 4 .......................................................................................0.61g
KH 2 PO 4 .......................................................................................0.39g
KCl..............................................................................................0.25g
Yeast extract................................................................................0.15g
MgSO 4 ·7H 2 O..............................................................................0.13g
Pyridine......................................................................................1.0mL
Trace elements solution .............................................................1.0mL
Trace Elements Solution:
Composition per liter:
FeSO 4 ·7H 2 O.............................................................................40.0mg
MnSO 4 ·4H 2 O...........................................................................40.0mg
ZnSO 4 ·7H 2 O............................................................................20.0mg
CuSO 4 ·5H 2 O..............................................................................5.0mg
Na 2 MoO 4 ·2H 2 O .........................................................................5.0mg
CoCl 2 ·6H 2 O ...............................................................................4.0mg
CaCl 2 ·2H 2 O................................................................................0.4mg
NaCl..............................................................................................1.0g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except pyridine, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature. In a fume hood, aseptically add 1.0mL of pyridine. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks. Use poly-
urethane foam closures to eliminate odors caused by volatilization of
pyridine.
Use: For the cultivation of Micrococcus luteus.