Pyridoxine Assay Medium

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pyridoxine Assay Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................30.0g
Ammonium tartrate.....................................................................10.0g
KH 2 PO 4 .........................................................................................5.0g
Sodium dihydrogen citrate............................................................4.0g
MgSO 4 ·7H 2 O................................................................................1.0g
CaCl 2 ·2H 2 O...................................................................................0.2g
NaCl..............................................................................................0.2g
Choline chloride..........................................................................0.01g
FeCl 3 ...........................................................................................0.01g
Thiamine·HCl .............................................................................0.01g
ZnSO 4 ·7H 2 O..............................................................................4.0mg
Nicotinic acid.............................................................................2.0mg
Calcium pantothenate ................................................................1.0mg
Riboflavin ..................................................................................1.0mg
p-Aminobenzoic acid.................................................................200μg
Biotin .............................................................................................8μg
pH 4.5 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Distribute into tubes in 5.0mL
volumes. Add standard solutions and test solutions to each tube. Bring
volume of each tube to 10.0mL with distilled/deionized water. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use: For the microbiological assay of pyridoxine using Neurospora
sitophila as the test organism.