Pyridoxine Y Medium

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pyridoxine Y Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................40.0g
(NH 4 ) 2 SO 4 ....................................................................................4.0g
L- Asparagine.................................................................................4.0g
KH 2 PO 4 .........................................................................................3.0g
MgSO 4 ·7H 2 O................................................................................1.0g
CaCl 2 ·2H 2 O ................................................................................0.49g
DL- Isoleucine...............................................................................0.04g
DL -Methionine ............................................................................0.04g
DL- Tryptophan.............................................................................0.04g
DL- Valine.....................................................................................0.04g
L- Histidine·HCl...........................................................................0.02g
Riboflavin...................................................................................0.02g
Biotin salt...................................................................................8.0mg
Inositol.......................................................................................5.0mg
FeSO 4 ·7H 2 O...............................................................................0.5mg
Calcium pantothenate ................................................................0.4mg
Nicotinic acid.............................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
H 3 BO 3 ........................................................................................0.2mg
KI...............................................................................................0.2mg
CuSO 4 ·5H 2 O............................................................................0.09mg
MnSO 4 ·H 2 O.............................................................................0.08mg
ZnSO 4 ·7H 2 O............................................................................0.08mg
(NH 4 ) 2 MoO 4 ............................................................................0.04mg
pH 4.4 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Distribute into tubes in 5.0mL
volumes. Add standard solutions and test solutions to each tube. Bring
volume of each tube to 10.0mL with distilled/deionized water. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use: For the microbiological assay of pyridoxine using Saccharomy-
ces uvarum as the test organism.