Pyrococcus endeavori Medium ES4

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pyrococcus endeavori Medium ES4

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 3.0L:

Solution A.....................................................................................1.0L
Solution B.....................................................................................1.0L
Solution C.....................................................................................1.0L
Solution A:
Composition per liter :
NaCl............................................................................................47.8g
Na 2 SO 4 ..........................................................................................8.0g
KCl................................................................................................1.4g
NaHCO 3 ........................................................................................0.4g
KBr ...............................................................................................0.2g
H 3 BO 3 .........................................................................................0.06g
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C.
Solution B:
Composition per liter :
MgCl 2 ·6H 2 O ...............................................................................21.6g
CaCl 2 ·2H 2 O ..................................................................................3.0g
SrCl 2 ·6H 2 O .................................................................................0.05g
Preparation of Solution B : Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C.
Solution C:
Composition per liter :
Sodium acetate............................................................................50.0g
NH 4 Cl .........................................................................................12.5g
K 2 HPO 4 .........................................................................................7.0g
Preparation of Solution C : Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C.
Preparation of Medium: Aseptically combine 1.0L of sterile solu-
tion A with 1.0L of sterile solution B and 1.0L of sterile solution C.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Pyrococcus endeavori.