Pyrococcus furiosus Medium

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pyrococcus furiosus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter :

NaCl............................................................................................13.8g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
Maltose .........................................................................................5.0g
MgSO 4 ..........................................................................................3.5g
MgCl 2 ..........................................................................................2.75g
KH 2 PO 4 .........................................................................................0.5g
CaCl 2 ...........................................................................................0.75g
KCl............................................................................................0.325g
NaBr.........................................................................................50.0mg
KI.............................................................................................50.0mg
H 3 BO 3 ......................................................................................15.0mg
SrCl 2 ...........................................................................................7.5mg
Citric acid...................................................................................5.0mg
Resazurin...................................................................................2.5mg
Mineral solution.......................................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Mineral Solution:
Composition per liter :
Nitrilotriacetic acid.......................................................................1.0g
MnSO 4 ..........................................................................................0.5g
FeCl 3 ·6H 2 O...................................................................................1.1g
Na 2 WO 4 ·2H 2 O..............................................................................0.3g
EDTA........................................................................................0.292g
NiCl 2 ·6H 2 O...................................................................................0.2g
CoSO 4 ·7H 2 O.................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.01g
Preparation of Mineral Solution:  Add nitrilotriacetic acid to
500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add
remaining components. Mix thoroughly. Add distilled/deionized water
to 1.0L. Adjust pH to 6.8.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use:  For the cultivation of high cell concentrations of Pyrococcus
furiosus.