Pyrolobus fumarii Medium (DSMZ Medium 792)

[所属分类:培养基配方] [发布时间:2021-7-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pyrolobus fumarii Medium
(DSMZ Medium 792)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl........................................................................................13.850g
MgSO 4 ·7H 2 O................................................................................3.5g
MgCl 2 ·6H 2 O................................................................................2.75g
KNO 3 .............................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
CaCl 2 ·2H 2 O...............................................................................0.375g
KCl............................................................................................0.325g
NaBr............................................................................................0.05g
H 3 BO 3 .......................................................................................0.015g
SrCl 2 ·6H 2 O.................................................................................7.5mg
Resazurin ...................................................................................1.0mg
Trace elements solution ...........................................................10.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
KI solution ...............................................................................0.05mL
pH 5.5 ± 0.2 at 25°C
Trace Elements Solution:
MgSO 4 ·7H 2 O................................................................................3.0g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CoSO 4 ·7H 2 O...............................................................................0.18g
FeSO 4 ·7H 2 O..................................................................................0.1g
CaCl 2 ·2H 2 O...................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O ..........................................................................0.01g
Na 2 WO 4 ·2H 2 O............................................................................0.01g
Na 2 SeO 3 ·5H 2 O.........................................................................0.30mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 1.0 with H 2 SO 4 .
Na 2 S·9H 2 O Solution :
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.3g
Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
KI Solution :
Composition per 10.0mL:
KI...............................................................................................5.0mg
Preparation of KI Solution: Add KI to distilled/deionized water
and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100%
N 2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Preparation of Medium: Add components, except Na 2 S·9H 2 O so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Cool to room temperature
while sparging with 80% H 2 + 20% CO 2 . Distribute into serum bottles
under 80% H 2 + 20% CO 2 , e.g., 20mL into 120mL serum bottles. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically
inject Na 2 S·9H 2 O solution, 0.2mL per 20mL medium. Mix thoroughly.
Adjust pH to 5.5. After inoculation pressurize vials to 2 bar overpres-
sure with 80% H 2 + 20% CO 2 gas mixture.
Use: For the cultivation of Pyrolobus fumarii.