Pyrrolidone Agar

[所属分类:培养基配方] [发布时间:2021-7-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pyrrolidone Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Noble agar...................................................................................21.0g
K 2 HPO 4 .......................................................................................5.65g
KH 2 PO 4 .......................................................................................2.95g
MgSO 4 ·7H 2 O................................................................................1.0g
Pyrrolidone carboxylic acid solution.......................................30.0mL
NaOH solution.........................................................................30.0mL
Trace metals...............................................................................6.3mL
Pyrrolidone Carboxylic Acid Solution:
Composition per 300.0mL:
Pyrrolidone carboxylic acid........................................................50.0g
Preparation of Pyrrolidone Carboxylic Acid Solution: Add
pyrrolidone carboxylic acid to distilled/deionized water and bring vol-
ume to 300.0mL. Mix thoroughly. Filter sterilize.
NaOH Solution:
Composition per 100.0mL:
NaOH............................................................................................5.0g
Preparation of NaOH Solution: Add NaOH to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Trace Metals:
Composition per 100.0mL:
FeSO 4 ·7H 2 O................................................................................0.18g
MnCl 2 ·2H 2 O ...............................................................................0.13g
CuSO 4 ·5H 2 O.................................................................................0.1g
ZnSO 4· 7H 2 O ...............................................................................0.02g
Preparation of Trace Metals: Add a few drops of H 2 SO 4 to dis-
tilled/deionized water to inhibit precipitate formation. Add compo-
nents to acidified distilled/deionized water and bring volume to
100.0mL. Mix thoroughly.
Preparation of Medium: Add components, except pyrrolidone car-
boxylic acid solution and NaOH solution, to distilled/deionized water
and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add 30.0mL of sterile pyrrolidone carboxylic
acid solution and 30.0mL of sterile NaOH solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Pseudomonas fluore-
scens.