Rabbit Heart Infusion Agar

[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]

Rabbit Heart Infusion Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from..........................................................500.0g
Agar............................................................................................15.0g
Tryptose......................................................................................10.0g
NaCl..............................................................................................5.0g
Rabbit blood, defibrinated.......................................................50.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except rabbit blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Aseptically add 50.0mL of sterile rabbit blood.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the culture and maintenance of Bartonella quintana.