Rabbit Laked Blood Agar

[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]

Rabbit Laked Blood Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Peptic digest of animal tissue .....................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Glucose.........................................................................................1.0g
NaHSO 3 ........................................................................................0.1g
Rabbit blood, laked..................................................................50.0mL
Hemin solution...........................................................................1.0mL
Vitamin K 1 solution...................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Hemin Solution:
Composition per 10.0mL:
Hemin ...........................................................................................0.5g
NaOH (1N solution).................................................................10.0mL
Preparation of Hemin Solution: Add hemin to 10.0mL of NaOH
solution. Mix thoroughly.
Vitamin K 1 Solution:
Composition per 20.0mL:
Vitamin K 1 (phytomenadione)......................................................0.2g
Ethanol (95% solution)............................................................20.0mL
Preparation of Vitamin K 1 Solution: Add vitamin K 1 to 20.0mL
of ethanol. Mix thoroughly.
Preparation of Medium: Add components, except vitamin K 1 so-
lution and laked rabbit blood, to distilled/deionized water and bring
volume to 849.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 1.0mL of sterile vitamin K 1 solution and 50.0mL of
sterile laked rabbit blood. Laked blood is prepared by freezing whole
blood overnight and thawing to room temperature. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and enhancement of pigment production of a
variety of anaerobic bacteria.