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培养基配方
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Rabbit Serum Medium (Rabbit Serum Bovine Serum Albumin Tween™ 80 Medium) (Rabbit Serum BSA Tween™ 80 Medium)
[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]
Rabbit Serum Medium
(Rabbit Serum Bovine Serum
Albumin Tween™ 80 Medium)
(Rabbit Serum BSA Tween™ 80 Medium)
Rabbit serum with supplements.............................................100.0mL
pH 7.4 ± 0.2 at 25°C
Basal Medium:
Composition per 900.0mL:
Na 2 HPO 4 .......................................................................................1.0g
NaCl..............................................................................................1.0g
KH 2 PO 4 .........................................................................................0.3g
Glycerol (10% solution).............................................................1.0mL
NH 4 Cl (25% solution)................................................................1.0mL
Sodium pyruvate (10% solution)...............................................1.0mL
Thiamine (0.5% solution)..........................................................1.0mL
Preparation of Basal Medium: Add components to distilled/de-
ionized water and bring volume to 900.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 20 min at 15 psi pressure–
121°C. Cool to 25°C.
Rabbit Serum with Supplements:
Composition per 106.0mL:
Rabbit serum..........................................................................100.0mL
L- Asparagine (3% solution) .......................................................5.0mL
MgCl 2 -CaCl 2 solution................................................................1.0mL
Preparation of Rabbit Serum with Supplements: Combine the
three solutions. Mix thoroughly. Filter sterilize.
MgCl 2 -CaCl 2 Solution:
Composition per 100.0mL:
CaCl 2 ·2H 2 O...................................................................................1.5g
MgCl 2 ·6H 2 O..................................................................................1.5g
Preparation of MgCl 2 -CaCl 2 Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Aseptically combine 900.0mL of cooled
sterile basal medium and 100.0mL of sterile rabbit serum with supple-
ments. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Leptospira species.
(Rabbit Serum Bovine Serum
Albumin Tween™ 80 Medium)
(Rabbit Serum BSA Tween™ 80 Medium)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Basal medium ........................................................................900.0mLRabbit serum with supplements.............................................100.0mL
pH 7.4 ± 0.2 at 25°C
Basal Medium:
Composition per 900.0mL:
Na 2 HPO 4 .......................................................................................1.0g
NaCl..............................................................................................1.0g
KH 2 PO 4 .........................................................................................0.3g
Glycerol (10% solution).............................................................1.0mL
NH 4 Cl (25% solution)................................................................1.0mL
Sodium pyruvate (10% solution)...............................................1.0mL
Thiamine (0.5% solution)..........................................................1.0mL
Preparation of Basal Medium: Add components to distilled/de-
ionized water and bring volume to 900.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 20 min at 15 psi pressure–
121°C. Cool to 25°C.
Rabbit Serum with Supplements:
Composition per 106.0mL:
Rabbit serum..........................................................................100.0mL
L- Asparagine (3% solution) .......................................................5.0mL
MgCl 2 -CaCl 2 solution................................................................1.0mL
Preparation of Rabbit Serum with Supplements: Combine the
three solutions. Mix thoroughly. Filter sterilize.
MgCl 2 -CaCl 2 Solution:
Composition per 100.0mL:
CaCl 2 ·2H 2 O...................................................................................1.5g
MgCl 2 ·6H 2 O..................................................................................1.5g
Preparation of MgCl 2 -CaCl 2 Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Aseptically combine 900.0mL of cooled
sterile basal medium and 100.0mL of sterile rabbit serum with supple-
ments. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Leptospira species.
