R8AH Medium (DSMZ Medium 651)

[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]

R8AH Medium
(DSMZ Medium 651)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Malic acid.....................................................................................2.5g
(NH 4 ) 2 SO 4 ..................................................................................1.25g
Yeast extract..................................................................................1.0g
K 2 HPO 4 .........................................................................................0.9g
KH 2 PO 4 .........................................................................................0.6g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.07g
EDTA..........................................................................................0.02g
Ferric citrate................................................................................0.01g
Vitamin solution.........................................................................7.5mL
Trace elements solution.............................................................1.0mL
pH 6.9 ± 0.2 at 25°C
Trace Elements Solution:
Composition per 100.0mL:
Ferric citrate..................................................................................0.3g
EDTA..........................................................................................0.05g
CaCl 2 ·2H 2 O ................................................................................0.02g
MnSO 4 ·H 2 O...............................................................................2.0mg
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O.................................................................2.0mg
H 3 BO 3 ........................................................................................1.0mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
ZnSO 4 ........................................................................................1.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Vitamin Solution:
Composition per liter:
Thiamine·HCl...............................................................................0.4g
Nicotinic acid................................................................................0.2g
Nicotinamide.................................................................................0.2g
Biotin.........................................................................................8.0mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add malic acid to 500.0mL of distilled/
deionized water. Adjust pH to 6.9 with NaOH. Add remaining compo-
nents. Bring volume to 1.0L with distilled/deionized water. Mix thor-
oughly. Adjust pH to 6.9. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Rhodopseudomonas palus-
tris, Rhodobacter sphaeroides, Rhodocyclus tenuis, Rhodopseudomonas
rutila, Rhodospirillum photometricum, and Rhodospirillum rubrum.