Rimler-Shotts Medium (RS Medium)

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rimler-Shotts Medium
(RS Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................13.5g
Na 2 S 2 O 3 ·5H 2 O..............................................................................6.8g
L- Ornithine·HCl.............................................................................6.5g
NaCl..............................................................................................5.0g
L- Lysine·HCl.................................................................................5.0g
Maltose..........................................................................................3.5g
Yeast extract..................................................................................3.0g
Sodium deoxycholate....................................................................1.0g
Ferric ammonium citrate...............................................................0.8g
L- Cysteine·HCl..............................................................................0.3g
Bromthymol Blue .......................................................................0.03g
Novobiocin solution.................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Novobiocin Solution:
Composition per 10.0mL:
Novobiocin.................................................................................5.0mg
Preparation of Novobiocin Solution: Add novobiocin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except novobiocin so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile no-
vobiocin solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the selective isolation, cultivation, and presumptive identifi-
cation of Aeromonas hydrophila and other Gram-negative bacteria
based on their ability to decarboxylate lysine and ornithine, ferment
maltose, and produce H 2 S. Maltose-fermenting bacteria appear as yel-
low colonies. Bacteria that produce lysine or ornithine decarboxylase
turn the medium greenish-yellow to yellow. Bacteria that produce H 2 S
appear as colonies with black centers.