RIOT Agar (Rice Infusion Oxgall Tween™ 80 Agar)

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

RIOT Agar
(Rice Infusion Oxgall Tween™ 80 Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Agar ............................................................................................10.0g
Oxgall..........................................................................................10.0g
Rice extract...................................................................................1.0L
Tween™ 80..............................................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Rice Extract:
Composition per liter:
Cream of rice cereal....................................................................10.0g
Preparation of Rice Extract: Add cream of rice cereal to 1.0L of
boiling tap water. Mix thoroughly. Filter quickly through cheesecloth.
Bring volume of filtrate to 1.0L with tap water.
Preparation of Medium: Combine components. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and differentiation of Candida albicans and
Candida stellatoidea from other Candida species based on chlamy-
dospore formation.