Rippey-Cabelli Agar (RC Agar)

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rippey-Cabelli Agar
(RC Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Meat peptone ................................................................................5.0g
Trehalose.......................................................................................5.0g
NaCl..............................................................................................3.0g
KCl................................................................................................2.0g
Yeast extract..................................................................................2.0g
Bromthymol Blue.......................................................................0.44g
MgSO 4 ·7H 2 O................................................................................0.2g
FeCl 3 ·6H 2 O...................................................................................0.1g
Sodium deoxycholate....................................................................0.1g
Ampicillin solution..................................................................10.0mL
Ethanol.....................................................................................10.0mL
pH 8.0 ± 0.2 at 25°C
Ampicillin Solution:
Composition per 10.0mL:
Ampicillin...................................................................................0.02g
Preparation of Ampicillin Solution: Add ampicillin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components—except sodium de-
oxycholate, ampicillin solution, and ethanol—to distilled/deionized
water and bring volume to 980.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add sodium deoxycholate, 10.0mL of sterile
ampicillin solution, and 10.0mL of ethanol. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation, cultivation, and differentiation of Aeromonas
species and Plesiomonas species from water samples using the mem-
brane filter method. This medium differentiates bacteria on the basis of
trehalose fermentation. Bacteria that ferment trehalose turn the med-
ium yellow.