Rippey-Cabelli HiVeg Agar Base with Ethanol and Ampicillin

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rippey-Cabelli HiVeg Agar Base
with Ethanol and Ampicillin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Plant hydrolysate No. 1.................................................................5.0g
Trehalose.......................................................................................5.0g
NaCl..............................................................................................3.0g
KCl................................................................................................2.0g
Yeast extract..................................................................................2.0g
MgSO 4 ..........................................................................................0.2g
Iron (III) chloride..........................................................................0.1g
Bromthymol Blue .......................................................................0.04g
Ampicillin solution..................................................................10.0mL
Ethanol.....................................................................................10.0mL
pH 8.0 ± 0.2 at 25°C
Source: This medium, without ampicillin solution and ethanol, is
available as a premixed powder from HiMedia.
Ampicillin Solution:
Composition per 10.0mL:
Ampicillin...................................................................................0.02g
Preparation of Ampicillin Solution: Add ampicillin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components—except ampicillin so-
lution and ethanol—to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 10.0mL of sterile ampicillin solution, and 10.0mL of ethanol. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation, cultivation, and differentiation of Aeromonas
species and Plesiomonas species from water samples using the mem-
brane filter method. For the differential and selective isolation of Aer-
omonas hydrophila species from water samples