RM Medium

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

RM Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition  per liter:

Glucose .......................................................................................20.0g
Agar ............................................................................................15.0g
Yeast extract................................................................................10.0g
KH 2 PO 4 .........................................................................................2.0g
Solution 1...............................................................................250.0mL
Solution 2...............................................................................250.0mL
Solution 3...............................................................................250.0mL
Solution 4...............................................................................250.0mL
pH 6.0 ± 0.2 at 25°C
Solution 1:
Composition per 250.0mL:
Glucose .......................................................................................20.0g
Preparation of Solution 1: Add glucose to distilled/deionized wa-
ter and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution 2:
Composition per 250.0mL:
Agar ............................................................................................15.0g
Preparation of Solution 2: Add agar to distilled/deionized water
and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution 3:
Composition per 250.0mL:
Yeast extract................................................................................10.0g
Preparation of Solution 3: Add yeast extract to distilled/deionized
water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution 4:
Composition per 250.0mL:
KH 2 PO 4 .........................................................................................2.0g
Preparation of Solution 4: Add KH 2 PO 4 to distilled/deionized wa-
ter and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Aseptically combine the four sterile solu-
tions. Mix thoroughly. Adjust pH to 6.0. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Zymomonas mobilis.