Rogosa Agar

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Rogosa Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium acetate............................................................................25.0g
Agar............................................................................................20.0g
Glucose.......................................................................................20.0g
Pancreatic digest of casein..........................................................10.0g
KH 2 PO 4 .........................................................................................6.0g
Yeast extract..................................................................................5.0g
Ammonium citrate........................................................................2.0g
Sorbitan monooleate.....................................................................1.0g
MgSO 4 ·7H 2 O............................................................................0.575g
MnSO 4 ·H 2 O................................................................................0.12g
FeSO 4 ·7H 2 O...............................................................................0.4mg
Acetic acid, glacial...................................................................1.32mL
pH 5.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components, except acetic acid, to
distilled/deionized water and bring volume to 998.7mL. Mix thorough-
ly. Gently heat and bring to boiling. Add glacial acetic acid. Mix thor-
oughly. Gently heat while stirring and bring to 90°–100°C for 2–3 min.
Do not autoclave. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the isolation, cultivation, and enumeration of lactobacilli,
especially from feces, saliva, vaginal specimens, and dairy products.