Rogosa SL HiVeg Broth

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Rogosa SL HiVeg Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium acetate............................................................................15.0g
Glucose.......................................................................................10.0g
Plant hydrolysate No. 1...............................................................10.0g
KH 2 PO 4 .........................................................................................6.0g
Arabinose......................................................................................5.0g
Saccharose ....................................................................................5.0g
Yeast extract..................................................................................5.0g
Ammonium citrate........................................................................2.0g
MgSO 4 ........................................................................................0.57g
MnSO 4 ........................................................................................0.12g
FeSO 4 ..........................................................................................0.03g
Acetic acid, glacial...................................................................1.32mL
Polysorbate 80 ...........................................................................1.0mL
pH 5.4 ± 0.2 at 25°C
Source: This medium, without acetic acid or polysorbate 80, is avail-
able as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Gently heat to 90–100°C.
Hold at temperature for 2–3 min. Do not autoclave.
Use: For the isolation, cultivation, and enumeration of lactobacilli,
especially from feces, saliva, vaginal specimens, and dairy products.