Rolled Oats Mineral Medium (DSMZ Medium 84)

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Rolled Oats Mineral Medium
(DSMZ Medium 84)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Rolled oats ..................................................................................20.0g
Agar ............................................................................................12.0g
Trace elements solution .............................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................0.1g
MnCl 2 ·4H 2 O..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Preparation of Medium: Add rolled oats to 500.0mL distilled/de-
ionized water. Gently heat and bring to boiling. Boil for 20 min. Filter.
Add agar to the filtrate and bring volume to 1.0L with distilled/deion-
ized water. Mix thoroughly. Add 1.0mL trace elements solution. Gen-
tly heat and bring to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the cultivation and maintenance of Actinomadura rubrobrunea,
Streptomyces chartreusis, Streptomyces aculeolatus, Streptomyces ther-
modiastaticus, Microbispora rosea, Micromonospora coerulea,
Thermoactinomyces vulgaris, Thermoactinomyces sacchari, Strepto-
sporangium album, and Planobispora rosea.