Rose Bengal Chloramphenicol Agar

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Rose Bengal Chloramphenicol Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Glucose .......................................................................................10.0g
Papaic digest of soybean meal......................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
Rose Bengal................................................................................0.05g
Chloramphenicol solution........................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Chloramphenicol Solution:
Composition per 10.0mL:
Chloramphenicol...........................................................................0.1g
Preparation of Chloramphenicol Solution: Add chlorampheni-
col to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except chlorampheni-
col solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°C. Aseptically add sterile
chloramphenicol solution. Mix thoroughly. Pour into sterile Petri dish-
es or distribute into sterile tubes.
Use: For the selective isolation, cultivation, and enumeration of yeasts
and molds from environmental specimens and foods.